| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.167337 |
| Chromosome: | chromosome 1 |
| Location: | 5653911 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g040300 | DNJ9 | Catechol O-methyltransferase; (1 of 1) 2.1.1.6 - Catechol O-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGTGGAGGTTGGGACAGGAAGCGAGCC |
| Internal bar code: | GGTCTGCTAGAATAGGTTTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 99.1589 |
| LEAP-Seq n confirming: | 8724 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTCACCATCGCTATCTG |
| Suggested primer 2: | GTGGCACTGACTCAGCAGAA |