Insertion junction: LMJ.RY0402.167348_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.167348
Chromosome:chromosome 16
Location:819506
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g647602 intron|outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGGGAGGAAGGGGAGGGGGTGGGTACAA
Internal bar code:ATAGAACTCAAGAATTGAATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:346
LEAP-Seq percent confirming:83.6735
LEAP-Seq n confirming:82
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CATGTGCGTTTAACCTCCCT
Suggested primer 2:GATGGTTTCAAAATGGGTGG