| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.167356 |
| Chromosome: | chromosome 16 |
| Location: | 1131047 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650050 | (1 of 2) PTHR12231//PTHR12231:SF112 - CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN // RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 14, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCTAGTTGCTATTGATTCGGTAATCCGC |
| Internal bar code: | CCGGTGTCGAGGCTCAGAGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 889 |
| LEAP-Seq percent confirming: | 99.8532 |
| LEAP-Seq n confirming: | 1360 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAAAGGCGTGATCGGTGTT |
| Suggested primer 2: | TGTGGCACTGAGAAGTGGAG |