| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.167370 |
| Chromosome: | chromosome 5 |
| Location: | 2186000 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234651 | (1 of 2) PTHR11122:SF12 - ALDOSE 1-EPIMERASE FAMILY PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCTCACAACTTGAAGCCGCAAAACACA |
| Internal bar code: | GGTCCTGGTCCTTGTTTCTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1164 |
| LEAP-Seq percent confirming: | 99.6796 |
| LEAP-Seq n confirming: | 7778 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGGGTGAGGGATAGGGT |
| Suggested primer 2: | CGTGGTGTGCAGTGTATTCC |