| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.167432 |
| Chromosome: | chromosome 12 |
| Location: | 8152590 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551100 | (1 of 1) PTHR36356:SF1 - EXPRESSED PROTEIN | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGATTCACCAACACAACGCAACATACCGC |
| Internal bar code: | GTTCGATGTCCTCGTACTCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1003 |
| LEAP-Seq percent confirming: | 98.519 |
| LEAP-Seq n confirming: | 3060 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAAATGAGCTGTTCCAA |
| Suggested primer 2: | GGGAAGAGCAGACAACAAGC |