| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.167434 |
| Chromosome: | chromosome_12 |
| Location: | 2569363 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g505350 | ACA2 | P-type ATPase/cation transporter, plasma membrane | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATCTTCTGGATGTTCCTCATCCTGTACGGC |
| Internal bar code: | GATTGTGCAATTGTCCCTGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 225 |
| LEAP-Seq percent confirming: | 99.6066 |
| LEAP-Seq n confirming: | 1519 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAACAGGCCCACAAAGAT |
| Suggested primer 2: | GAGAGACAACGGAGTGAGCC |