| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.167447 |
| Chromosome: | chromosome 2 |
| Location: | 4094245 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g097600 | FKM2 | DNA methylase in the FkbM family; (1 of 13) PF05050 - Methyltransferase FkbM domain (Methyltransf_21) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGTGAGGACGGCTCACAGCTGCAGCGCC |
| Internal bar code: | AGTCTTGCTCCCAATGATTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 697 |
| LEAP-Seq percent confirming: | 99.6191 |
| LEAP-Seq n confirming: | 2354 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAACGTGGTACAGCAGTG |
| Suggested primer 2: | ACACAAAGTGGGGTTGGAAG |