| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.167452 |
| Chromosome: | chromosome 9 |
| Location: | 6490068 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g407373 | (1 of 2) 2.3.1.128 - Ribosomal-protein-alanine N-acetyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTCCAACTGCAATCTCCAAAAGAGCTTT |
| Internal bar code: | CGCTTTACCGGGTCTAGGATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 872 |
| LEAP-Seq percent confirming: | 90.0831 |
| LEAP-Seq n confirming: | 1735 |
| LEAP-Seq n nonconfirming: | 191 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTGAAGCAGGACACGGAC |
| Suggested primer 2: | CCTGACACCGATTACCGACT |