| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.167452 |
| Chromosome: | chromosome 9 |
| Location: | 6490071 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g407373 | (1 of 2) 2.3.1.128 - Ribosomal-protein-alanine N-acetyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCCAAGAGCGCGACGGTGTCGTGGAGCA |
| Internal bar code: | CGATCTAATCTGCAATGTACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 374 |
| LEAP-Seq percent confirming: | 85.6581 |
| LEAP-Seq n confirming: | 15690 |
| LEAP-Seq n nonconfirming: | 2627 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTGAAGCAGGACACGGAC |
| Suggested primer 2: | CCTGACACCGATTACCGACT |