| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.167466 |
| Chromosome: | chromosome 9 |
| Location: | 2357358 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392000 | (1 of 3) PF09763 - Exocyst complex component Sec3 (Sec3_C) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTCCAGCAGGTGGGCGGGGTTGTGCTCG |
| Internal bar code: | GTAGGGCTATTTCGACATCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 19 |
| LEAP-Seq percent confirming: | 70.354 |
| LEAP-Seq n confirming: | 318 |
| LEAP-Seq n nonconfirming: | 134 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCGAGGCAAAGTATGAGG |
| Suggested primer 2: | CCTACCCTCTGAAGACGCTG |