| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.167467 |
| Chromosome: | chromosome 12 |
| Location: | 7275749 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557850 | (1 of 7) IPR000048//IPR027417 - IQ motif, EF-hand binding site // P-loop containing nucleoside triphosphate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGATTTTGTGAGGTGCGTCTCAGCTGAG |
| Internal bar code: | AGAGGATCCTTAGGGGCAGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 677 |
| LEAP-Seq percent confirming: | 99.9023 |
| LEAP-Seq n confirming: | 1023 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAGCTAAGGGCGTTGCTGA |
| Suggested primer 2: | CGTTGTACGGTGTCCATCTG |