| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.167476 |
| Chromosome: | chromosome 3 |
| Location: | 4488078 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176200 | (1 of 1) PTHR22904//PTHR22904:SF364 - TPR REPEAT CONTAINING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCAATGCCGTGCAGCTCCCGCAATCGCT |
| Internal bar code: | ATGCGCAGGCTTCACGGGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 715 |
| LEAP-Seq percent confirming: | 97.8877 |
| LEAP-Seq n confirming: | 1900 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGTACGAGAGCAGATCC |
| Suggested primer 2: | CCAGAGGCTAGTTGCAGAGG |