| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.167482 |
| Chromosome: | chromosome 17 |
| Location: | 3506149 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725100 | (1 of 1) PTHR11390:SF20 - DNA TOPOISOMERASE 3-BETA-1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGTTGCAGCGGTTGCAGTCCAGACGCG |
| Internal bar code: | ACTGTGAGGGCTTGGTACGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 504 |
| LEAP-Seq percent confirming: | 78.4148 |
| LEAP-Seq n confirming: | 1573 |
| LEAP-Seq n nonconfirming: | 433 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGACAAAGAAGCTGGAGG |
| Suggested primer 2: | AGGGAGTCATGAAGGTGTGG |