| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.167483 |
| Chromosome: | chromosome 16 |
| Location: | 414852 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g693300 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGGGGCCGACGGAAGGATAGGGACGAG |
| Internal bar code: | TCTGGGCTTAGTCGTTAGCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1056 |
| LEAP-Seq percent confirming: | 98.5773 |
| LEAP-Seq n confirming: | 4088 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTTCGGTAACATTGTGGA |
| Suggested primer 2: | TACTCGAGTCCGAAGCACCT |