| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.167502 |
| Chromosome: | chromosome 7 |
| Location: | 604379 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g316850 | MCM4 | (1 of 1) K02212 - DNA replication licensing factor MCM4 (MCM4, CDC54); Minichromosome maintenance protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCCCTTCAGCATTGGAGGAACATCCTT |
| Internal bar code: | TTCACGGGCCAGTCGTTGAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 708 |
| LEAP-Seq percent confirming: | 99.4536 |
| LEAP-Seq n confirming: | 1456 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTATGATGCCAGAGGCGT |
| Suggested primer 2: | AAAGTGCAGGCAAGGAAAGA |