| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.167502 |
| Chromosome: | chromosome 7 |
| Location: | 604387 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g316850 | MCM4 | (1 of 1) K02212 - DNA replication licensing factor MCM4 (MCM4, CDC54); Minichromosome maintenance protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGTTGCGACAGGAAAAGCACCATGAGCT |
| Internal bar code: | GAGATTTCCCGGCTATGAGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 388 |
| LEAP-Seq percent confirming: | 78.3549 |
| LEAP-Seq n confirming: | 4239 |
| LEAP-Seq n nonconfirming: | 1171 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTATGATGCCAGAGGCGT |
| Suggested primer 2: | AAAGTGCAGGCAAGGAAAGA |