Insertion junction: LMJ.RY0402.167533_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.167533
Chromosome:chromosome 4
Location:2357478
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g220850 (1 of 1) 3.6.3.34//3.6.3.43//3.6.3.44 - Iron-chelate-transporting ATPase // Peptide-transporting ATPase // Xenobiotic-transporting ATPase / Steroid-transporting ATPase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGACTTCATTACCGTGCATCGATTGGGGA
Internal bar code:CAAACGCAACGGGGGTGGCAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:373
LEAP-Seq percent confirming:99.3533
LEAP-Seq n confirming:3687
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTTGTGGTGTAGGTTGTG
Suggested primer 2:GAGTGGGTGCTGGTTTTGTT