| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.167534 |
| Chromosome: | chromosome 2 |
| Location: | 6061326 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g112750 | (1 of 19) PTHR31251:SF1 - SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 10-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAATCAACAAGTTCCACATCCTTGCCGC |
| Internal bar code: | GATGTTTGCCCTCTGCCTCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 615 |
| LEAP-Seq percent confirming: | 99.5356 |
| LEAP-Seq n confirming: | 3215 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCGAACAAAAGGAGTTGC |
| Suggested primer 2: | TGGCAGAGAACAGCTCCAC |