| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.167544 |
| Chromosome: | chromosome 1 |
| Location: | 5355494 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037550 | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTTGTGTCGGTCGGCAGCGCTGACGGC |
| Internal bar code: | GCCGCTTGGGGTAGGTCGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 742 |
| LEAP-Seq percent confirming: | 51.1111 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 88 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACCTGGTCTTGCACACA |
| Suggested primer 2: | AACAGGTTAGGGAGGCTCGT |