| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.167567 |
| Chromosome: | chromosome 9 |
| Location: | 391953 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g404904 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACCGTGCACTATATGCACGTATGATGAA |
| Internal bar code: | ACGGGTTCCCTAAGGCGCCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 935 |
| LEAP-Seq percent confirming: | 98.3279 |
| LEAP-Seq n confirming: | 5175 |
| LEAP-Seq n nonconfirming: | 88 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTTCTGCAAGGAGAAGG |
| Suggested primer 2: | GTAAGGAGCAGTTGGCAAGC |