| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.167576 |
| Chromosome: | chromosome 9 |
| Location: | 3697705 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390875 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGCCTAGGTTCCCTCTTGGGCCCCCAG |
| Internal bar code: | CTCGCCGGGCCGAGCGCTACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1162 |
| LEAP-Seq percent confirming: | 99.711 |
| LEAP-Seq n confirming: | 4485 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGCAAGTCAGGGGTTACT |
| Suggested primer 2: | ATGCCTTCCCAAGTGTTCTG |