| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.167587 |
| Chromosome: | chromosome 13 |
| Location: | 1956450 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g576500 | (1 of 1) PTHR35698//PTHR35698:SF1 - FAMILY NOT NAMED // DNA-BINDING PROTEIN RHL1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAGAACGACGGCTGATAGAGAAATGCCG |
| Internal bar code: | AGCACTTACTCCTATCTCTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 281 |
| LEAP-Seq percent confirming: | 95.4357 |
| LEAP-Seq n confirming: | 230 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAATAAAGCTTCACCGCGA |
| Suggested primer 2: | ATGAAATCATCCGGTACCCA |