| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.167602 |
| Chromosome: | chromosome 14 |
| Location: | 3208713 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629150 | CYG4 | Adenylate/guanylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCCAAGCAGGCTTGCACTTCAGTGAGT |
| Internal bar code: | GTTACATGCATAAGCAGGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 646 |
| LEAP-Seq percent confirming: | 86.3578 |
| LEAP-Seq n confirming: | 1342 |
| LEAP-Seq n nonconfirming: | 212 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGGATATGTTTTTGCCGC |
| Suggested primer 2: | TAGTTGCAGTCCTTGGCTCC |