Insertion junction: LMJ.RY0402.167612_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.167612
Chromosome:chromosome 2
Location:3241483
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g095076 ROC39,MFT10 Major facilitator superfamily transporter; (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCGACGCAAGTCCTGCTCAGTGAGAGAC
Internal bar code:ACCGTGAATAAGATTTGAAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1355
LEAP-Seq percent confirming:99.6514
LEAP-Seq n confirming:11150
LEAP-Seq n nonconfirming:39
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTGCTTGCACAGTATGAT
Suggested primer 2:CCATCAACAACACCAACAGC