Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.167624 |
Chromosome: | chromosome_17 |
Location: | 4497360 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g732500 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCGTACGTGGCACGCACCTTCGTGGCCACC |
Internal bar code: | TTTTAACTATTTCCATAGGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 942 |
LEAP-Seq percent confirming: | 90.968 |
LEAP-Seq n confirming: | 7997 |
LEAP-Seq n nonconfirming: | 794 |
LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCCAGCGAGATGAAGGAGTT |
Suggested primer 2: | ACTCAAGCGCGCTGAATTAT |