Insertion junction: LMJ.RY0402.167629_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.167629
Chromosome:chromosome 6
Location:6430957
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g292500 CYN23b,CYN23 Cyclophilin 23; (1 of 1) PTHR11071//PTHR11071:SF17 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // CYCLOPHILIN-LIKE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FAMILY PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTGCAGGAACACCCGCAGGGGAGCACTG
Internal bar code:GTGCGCGCCGACGTCCGAAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:636
LEAP-Seq percent confirming:99.7644
LEAP-Seq n confirming:5082
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GTACCAGTAGGTGGCCTGGA
Suggested primer 2:GGAGATGTCAAGGTCGGTGT