| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.167637 |
| Chromosome: | chromosome 16 |
| Location: | 6358033 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674050 | ADH12 | Alcohol dehydrogenase; (1 of 1) 1.6.5.5 - NADPH:quinone reductase / Zeta-crystallin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCCCGGCCACTCGGTTCAGTGTCCATT |
| Internal bar code: | CAGGAGGACTGGCGGCGGAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 79 |
| LEAP-Seq percent confirming: | 97.4026 |
| LEAP-Seq n confirming: | 300 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCCTCGCTTACCAGAACA |
| Suggested primer 2: | TATAAACCCCCTCCCACTCC |