| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.167658 |
| Chromosome: | chromosome 6 |
| Location: | 6751188 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g294650 | AGT1 | Alanine-glyoxylate transaminase; (1 of 2) K00830 - alanine-glyoxylate transaminase / serine-glyoxylate transaminase / serine-pyruvate transaminase (AGXT) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCTACACACCCCTACATACACACCTGGT |
| Internal bar code: | CAGAGCCTCCAGGAGCTAAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 213 |
| LEAP-Seq percent confirming: | 99.7297 |
| LEAP-Seq n confirming: | 369 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATCTCTTGCATTCCACT |
| Suggested primer 2: | GTACGAGTGTGGGATGCCTT |