Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.167660 |
Chromosome: | chromosome 17 |
Location: | 3352513 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g723500 | (1 of 1) 3.6.3.30 - Fe(3+)-transporting ATPase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGGCCTAAGGCCGTGGCTCGAGCTAGC |
Internal bar code: | AGACATTCGTACGATGGATCCCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 944 |
LEAP-Seq percent confirming: | 93.2716 |
LEAP-Seq n confirming: | 2315 |
LEAP-Seq n nonconfirming: | 167 |
LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCAAGTGAAGAGCGCGTATG |
Suggested primer 2: | GGTGACCACGTTCCAGTTCT |