| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.167667 |
| Chromosome: | chromosome 13 |
| Location: | 3552184 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588100 | CYN19C,ROC2,CYN5,CYN19-3 | (1 of 1) K09565 - peptidyl-prolyl isomerase F (cyclophilin D) (PPIF); Cyclophilin 19-3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCCCTACATCCCTATCCACGCCTCGTGC |
| Internal bar code: | TTGTTCGGCGCGCTAAGGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 130 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTCGTAAAATGGTGCCT |
| Suggested primer 2: | GGGGCAAACTGCCATAAGTA |