| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.167693 |
| Chromosome: | chromosome 12 |
| Location: | 411390 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493903 | CSC7 | Chlamydomonadale-specific family C protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCGTCAGAGCCTCCATGCTGCTACGACA |
| Internal bar code: | AGCTTCTTTGCGCGACACATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 454 |
| LEAP-Seq percent confirming: | 99.8242 |
| LEAP-Seq n confirming: | 1704 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGAGGAGCTAAGGGCTG |
| Suggested primer 2: | CCGTGTGTTTGGTTGAGATG |