| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.167696 |
| Chromosome: | chromosome 12 |
| Location: | 4915257 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g525450 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTGTGGAAGTAGGGATGCGGGAGGTCG |
| Internal bar code: | TAGGTCCGCAGGGCCGCGAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1015 |
| LEAP-Seq percent confirming: | 96.0155 |
| LEAP-Seq n confirming: | 7687 |
| LEAP-Seq n nonconfirming: | 319 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTACTCCTTGCCCATCAC |
| Suggested primer 2: | AAAGGGCATGCTCGTCATAG |