Insertion junction: LMJ.RY0402.167699_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.167699
Chromosome:chromosome 10
Location:4063089
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g449350 (1 of 1) PTHR11717//PTHR11717:SF2 - LOW MOLECULAR WEIGHT PROTEIN TYROSINE PHOSPHATASE // PEPTIDYL-TRNA HYDROLASE PTRHD1-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTTCTTGAAGTTGCATGGCGATCGCACAA
Internal bar code:CCATTCCGGGGTCATCCAATCC

Confirmation - LEAP-Seq

LEAP-Seq distance:43
LEAP-Seq percent confirming:90.5263
LEAP-Seq n confirming:86
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GACGCTCGTTCAACATGCTA
Suggested primer 2:CTAACTAGCACACGTCGCCA