| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.167705 |
| Chromosome: | chromosome 3 |
| Location: | 7311481 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206850 | (1 of 2) IPR004911//IPR012336 - Gamma interferon inducible lysosomal thiol reductase GILT // Thioredoxin-like fold | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTCCGCGCAGCCGCTGGGCGTCACCTGC |
| Internal bar code: | GACAAACAATCTGCTGACGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 762 |
| LEAP-Seq percent confirming: | 99.3677 |
| LEAP-Seq n confirming: | 5500 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGGCTTTACCGAAATGAA |
| Suggested primer 2: | TACGGATTTGTAGGCCTTGC |