| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.167708 |
| Chromosome: | chromosome 2 |
| Location: | 1437238 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g083600 | (1 of 1) PTHR23305//PTHR23305:SF7 - GTP-BINDING PROTEIN-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCGTTCTGTTCCCTTCAGCTTTGACCCG |
| Internal bar code: | CGACCCGTATCCTAGGTCTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 406 |
| LEAP-Seq percent confirming: | 99.5384 |
| LEAP-Seq n confirming: | 3666 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGTCAGGCTTGAGGGACG |
| Suggested primer 2: | TGCTCGAGATGCATCAATTC |