| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.167712 |
| Chromosome: | chromosome 16 |
| Location: | 7124644 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680454 | (1 of 8) PF13417 - Glutathione S-transferase, N-terminal domain (GST_N_3) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAACTCGCCAGGCGGTGAGACGCGATTAC |
| Internal bar code: | GACACATGCAGGGGCTTTGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 179 |
| LEAP-Seq percent confirming: | 98.1468 |
| LEAP-Seq n confirming: | 2701 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTCACCTGTGACCTTGCT |
| Suggested primer 2: | TTATGCTCGACAGCAACCTG |