| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.167725 |
| Chromosome: | chromosome 1 |
| Location: | 4578357 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g031300 | CDPK4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCCTCCTGCCTCCATCTCCTCCCGCCT |
| Internal bar code: | GACACGGCGCAGAGATCCGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 633 |
| LEAP-Seq percent confirming: | 99.6198 |
| LEAP-Seq n confirming: | 1048 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAATGCTGCTTGTGGACCC |
| Suggested primer 2: | GGCTGCACACACATCCATAC |