| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.167775 |
| Chromosome: | chromosome 12 |
| Location: | 1185262 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489850 | STPK10,STK10 | Serine/threonine protein kinase; (1 of 107) PF00069//PF07714 - Protein kinase domain (Pkinase) // Protein tyrosine kinase (Pkinase_Tyr) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGAACGTTGTCGACCACTGGAAGCATCG |
| Internal bar code: | TAGCCGGATAATGACAGTGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 988 |
| LEAP-Seq percent confirming: | 91.4112 |
| LEAP-Seq n confirming: | 4353 |
| LEAP-Seq n nonconfirming: | 409 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGGCAGCTATACGGAATG |
| Suggested primer 2: | GCTTCCCTGTTGTCCATTGT |