| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.167778 |
| Chromosome: | chromosome 10 |
| Location: | 136212 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g418700 | DMA4 | (1 of 1) PF10237 - Probable N6-adenine methyltransferase (N6-adenineMlase); Putative DNA methylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGATTGGCAAATACTATCTGGGGCGGGGG |
| Internal bar code: | ACTGCGAGTTTAGCAGTACTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1011 |
| LEAP-Seq percent confirming: | 93.721 |
| LEAP-Seq n confirming: | 1821 |
| LEAP-Seq n nonconfirming: | 122 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGCAGCTTGTACTGTTTG |
| Suggested primer 2: | GATGCAATGGTTGGGGATAC |