| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.167779 |
| Chromosome: | chromosome 10 |
| Location: | 2194322 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433800 | CSE21 | Predicted protein of CSE family | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCGTGCTGACCGTCCAGCTGCTGCCCT |
| Internal bar code: | ACTGAGCCTACGGTGCGGCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 945 |
| LEAP-Seq percent confirming: | 44.2408 |
| LEAP-Seq n confirming: | 676 |
| LEAP-Seq n nonconfirming: | 852 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAAGCGGCTTGCTTTTGTC |
| Suggested primer 2: | CTTCTCCAGCGTCATCAACA |