| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.167779 |
| Chromosome: | chromosome 12 |
| Location: | 8637399 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546850 | (1 of 4) PTHR11266//PTHR11266:SF42 - PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17 // SUBFAMILY NOT NAMED; Putative peroxisomal membrane protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGCTGTCGCGGCGAGAGGGCTTGACCGA |
| Internal bar code: | GAAGGACGCCACCTTAGCCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 714 |
| LEAP-Seq percent confirming: | 99.7033 |
| LEAP-Seq n confirming: | 336 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTTTACCTGACTTCCAG |
| Suggested primer 2: | TGCCTCCCAAGTAACCATTC |