| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.167786 |
| Chromosome: | chromosome 5 |
| Location: | 1117031 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245901 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGCTCTGATGGCGCATTGCGAGAGCCG |
| Internal bar code: | AAGGTTAGGGTGCCGGGCGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 480 |
| LEAP-Seq percent confirming: | 97.9487 |
| LEAP-Seq n confirming: | 382 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGTAACGCTACACGGCAG |
| Suggested primer 2: | GCCACGTCTACCACCATCTT |