| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.167790 |
| Chromosome: | chromosome 6 |
| Location: | 8991600 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g311700 | FAP349 | Leucine-Rich Repeat Flagellar Associated Protein 349 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACATACAGTGCCCTCCACGGGTGCGACA |
| Internal bar code: | GAAGGTGTGAGGCTGGTCCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 302 |
| LEAP-Seq percent confirming: | 99.7262 |
| LEAP-Seq n confirming: | 1457 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATTCTGAACGCTCTCGTC |
| Suggested primer 2: | ACTGTTCACAGGCTGACACG |