Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.167790 |
Chromosome: | chromosome_6 |
Location: | 8991600 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g311700 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CGACATACAGTGCCCTCCACGGGTGCGACA |
Internal bar code: | GAAGGTGTGAGGCTGGTCCCGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 302 |
LEAP-Seq percent confirming: | 99.7262 |
LEAP-Seq n confirming: | 1457 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGATTCTGAACGCTCTCGTC |
Suggested primer 2: | ACTGTTCACAGGCTGACACG |