Insertion junction: LMJ.RY0402.167790_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.167790
Chromosome:chromosome_8
Location:4151436
Confidence (%):73
Locus disrupted Locus common name Defline Orientation Feature
Cre08.g379900 antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):GTGCTTGGCGGCGGCGGGACAGGCGGCGGG
Internal bar code:AGATGTGCGTCCCCCTAATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:138
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TTTTCCTGTGCTTTCCTGCT
Suggested primer 2:TTGTAACGACAGCTTGCGAC