| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.167791 |
| Chromosome: | chromosome 5 |
| Location: | 1430007 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g244100 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCGAGAGGACGCCTTCCACAACCCGCA |
| Internal bar code: | GCATTCAGCATACGGGCGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 766 |
| LEAP-Seq percent confirming: | 98.912 |
| LEAP-Seq n confirming: | 2000 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTAAAGAGCAGCTCATCC |
| Suggested primer 2: | CTGTCGTACCACCTCAGCG |