| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.167800 |
| Chromosome: | chromosome 11 |
| Location: | 1292806 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467710 | VSP1 | Hydroxyproline-rich cell wall glycoprotein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAATACGAACATGTGTGTGTTCACGTGC |
| Internal bar code: | GATGTCCCTATGCTGTATATACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 751 |
| LEAP-Seq percent confirming: | 89.8734 |
| LEAP-Seq n confirming: | 2201 |
| LEAP-Seq n nonconfirming: | 248 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACGCACAACTAACAGCAT |
| Suggested primer 2: | AAACCAGGTCCTGTTATGCG |