| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.167800 |
| Chromosome: | chromosome 11 |
| Location: | 1292810 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467710 | VSP1 | Hydroxyproline-rich cell wall glycoprotein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAATTGCAGCGGGCCGGATGAGCCTGAG |
| Internal bar code: | GACGTGAGTACCAGTCATGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1146 |
| LEAP-Seq percent confirming: | 97.4318 |
| LEAP-Seq n confirming: | 607 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACGCACAACTAACAGCAT |
| Suggested primer 2: | AAACCAGGTCCTGTTATGCG |