| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.167812 |
| Chromosome: | chromosome_2 |
| Location: | 7346753 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g145100 | FAP39 | P-type ATPase/cation transporter, flagellar associated | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCTCGTCCAGCAGCTCGGGGTAGGGGTTCT |
| Internal bar code: | GGGAATGAAGGATTGCCTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 546 |
| LEAP-Seq percent confirming: | 99.4344 |
| LEAP-Seq n confirming: | 879 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACTTCAGCTCCATCGTCA |
| Suggested primer 2: | CCGCAGTAGCCCATAATGTT |