Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.167812 |
Chromosome: | chromosome_2 |
Location: | 7346754 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g145100 | FAP39 | P-type ATPase/cation transporter, flagellar associated | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATGCCACACGGCCGCTCCGAGCCCATCATC |
Internal bar code: | CTGCTGAGTCCGGGGGGAGTAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1089 |
LEAP-Seq percent confirming: | 67.1931 |
LEAP-Seq n confirming: | 1872 |
LEAP-Seq n nonconfirming: | 914 |
LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAACTTCAGCTCCATCGTCA |
Suggested primer 2: | CCGCAGTAGCCCATAATGTT |