Insertion junction: LMJ.RY0402.167819_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.167819
Chromosome:chromosome_12
Location:7517810
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre12.g555950 DHC11 Putative flagellar inner arm dynein heavy chain, novel minor typ sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):CGAACCTGCTCCCAGGTTGGACCCATTGGA
Internal bar code:TATGGTGAAGGTGATATATCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:252
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CATGTTCGTGGTCAGGTACG
Suggested primer 2:ACAGTGTCACAAGGAAGGGC